NAME

Barcode_split - simple tool to split sequencing summary report in per barcodes

DESCRIPTION

usage: Barcode_split [-h] [--version] --summary_file [SUMMARY_FILE ...]
[--barcode_file [BARCODE_FILE ...]]
[--output_dir OUTPUT_DIR] [--output_unclassified] [--min_barcode_percent MIN_BARCODE_PERCENT] [-v | -q]
Barcode_split is a simple tool to split sequencing summary report in per barcodes

optional arguments:

-h, --help
show this help message and exit
--version
show program's version number and exit
--summary_file [SUMMARY_FILE ...], -f [SUMMARY_FILE ...]
Path to a sequencing_summary generated by Albacore 1.0.0 + (read_fast5_basecaller.py) / Guppy 2.1.3+ (guppy_basecaller). One can also pass multiple space separated file paths or a UNIX style regex matching multiple files
--barcode_file [BARCODE_FILE ...], -b [BARCODE_FILE ...]
Path to the barcode_file generated by Guppy 2.1.3+ (guppy_barcoder) or Deepbinner 0.2.0+. One can also pass multiple space separated file paths or a UNIX style regex matching multiple files
--output_dir OUTPUT_DIR, -o OUTPUT_DIR
Folder where to output split barcode data (default: current dir
--output_unclassified, -u
If given, unclassified barcodes are also written in a file. By default they are skiped
--min_barcode_percent MIN_BARCODE_PERCENT, -p MIN_BARCODE_PERCENT
Minimal percent of total reads to retain barcode label. If below, the barcode value is set as `unclassified` (default: 0.1)
-v, --verbose
Increase verbosity
-q, --quiet
Reduce verbosity

AUTHOR


This manpage was written by Nilesh Patra for the Debian distribution and
can be used for any other usage of the program.