NAME

NanoStat - manual page for NanoStat 1.4.0

DESCRIPTION

usage: NanoStat [-h] [-v] [-o OUTDIR] [-p PREFIX] [-n NAME] [-t N] [--tsv]
[--barcoded] [--readtype {1D,2D,1D2}] ( --fastq file [file ...] | --fasta file [file ...] | --summary file [file ...] | --bam file [file ...] | --ubam file [file ...] | --cram file [file ...] | --feather file [file ...])
Calculate statistics of long read sequencing dataset.

General options:

-h, --help
show the help and exit
-v, --version
Print version and exit.
-o, --outdir OUTDIR
Specify directory for output, only in combination with -n.
-p, --prefix PREFIX
Specify an optional prefix to be used for the output file.
-n, --name NAME
Specify a filename/path for the output, stdout is the default.
-t, --threads N
Set the allowed number of threads to be used by the script.
--tsv
Output the stats as a properly formatted TSV.

Input options.:

--barcoded
Use if you want to split the summary file by barcode
--readtype {1D,2D,1D2}
Which read type to extract information about from summary. Options are 1D, 2D, 1D2

Input data sources, one of these is required.:

--fastq file [file ...]
Data is in one or more (compressed) fastq file(s).
--fasta file [file ...]
Data is in one or more (compressed) fasta file(s).
--summary file [file ...]
Data is in one or more (compressed) summary file(s)generated by albacore or guppy.
--bam file [file ...]
Data is in one or more sorted bam file(s).
--ubam file [file ...]
Data is in one or more unmapped bam file(s).
--cram file [file ...]
Data is in one or more sorted cram file(s).
--feather file [file ...]
Data is in one or more feather file(s).

EXAMPLES:

NanoStat --fastq reads.fastq.gz --outdir statreports NanoStat --summary sequencing_summary1.txt sequencing_summary2.txtsequencing_summary3.txt --readtype 1D2 NanoStat --bam alignment.bam alignment2.bam

SEE ALSO

The full documentation for NanoStat is maintained as a Texinfo manual. If the info and NanoStat programs are properly installed at your site, the command
info NanoStat
should give you access to the complete manual.