samtools-calmd - calculates MD and NM tags
samtools calmd [
-Eeubr] [
-C capQcoef]
aln.bam
ref.fasta
Generate the MD tag. If the MD tag is already present, this command will give a
warning if the MD tag generated is different from the existing tag. Output SAM
by default.
Calmd can also read and write CRAM files although in most cases it is pointless
as CRAM recalculates MD and NM tags on the fly. The one exception to this case
is where both input and output CRAM files have been / are being created with
the
no_ref option.
Note that some aligners do not include sequence or confidence values in
secondary and supplementary alignment records. Where this happens in SAM
files, a “*” character will be seen in the
SEQ and
QUAL columns. These records will be skipped, as it is not possible to
recalculate the MD and NM tags without access to the query sequence.
samtools calmd will emit a warning if any records have been skipped for
this reason.
- -A
- When used jointly with -r this option overwrites the
original base quality.
- -e
- Convert a the read base to = if it is identical to the
aligned reference base. Indel caller does not support the = bases at the
moment.
- -u
- Output uncompressed BAM
- -b
- Output compressed BAM
-
-C INT
- Coefficient to cap mapping quality of poorly mapped reads.
See the mpileup command for details. [0]
- -r
- Compute the BQ tag (without -A) or cap base quality by BAQ
(with -A).
- -E
- Extended BAQ calculation. This option trades specificity
for sensitivity, though the effect is minor.
- --no-PG
- Do not add a @PG line to the header of the output
file.
-
-@, --threads INT
- Number of input/output compression threads to use in
addition to main thread [0].
- o
- Dump BAQ applied alignment for other SNP callers:
samtools calmd -bAr aln.bam > aln.baq.bam
It adds and corrects the NM and MD tags at the same time. The
calmd command also comes with the -C option, the same as the
one in mpileup. Apply if it helps.
Written by Heng Li from the Sanger Institute.
samtools(1),
samtools-mpileup(1)
Samtools website: <
http://www.htslib.org/>